Vrije Universiteit Brussel






Research Papers

1. Cortvrindt R, Smitz J, Van Steirteghem AC. In-vitro maturation, fertilization and embryo development of immature oocytes from early preantral follicles from prepuberal mice in a simplified culture system. Hum Reprod. 1996 Dec;11(12):2656-66.

2. Cortvrindt R, Smitz J, Van Steirteghem AC. A morphological and functional study of the effect of slow freezing followed by complete in-vitro maturation of primary mouse ovarian follicles. Hum Reprod. 1996 Dec;11(12):2648-55.

3. Smitz J, Cortvrindt R, Van Steirteghem AC. Normal oxygen atmosphere is essential for the solitary long-term culture of early preantral mouse follicles. Mol Reprod Dev. 1996 Dec;45(4):466-75.

4. Cortvrindt R, Smitz J, Van Steirteghem AC. Assessment of the need for follicle stimulating hormone in early preantral mouse follicle culture in vitro. Hum Reprod. 1997 Apr;12(4):759-68.

5. Cortvrindt R, Hu Y, Smitz J. Recombinant luteinizing hormone as a survival and differentiation factor increases oocyte maturation in recombinant follicle stimulating hormone-supplemented mouse preantral follicle culture. Hum Reprod. 1998 May;13(5):1292-302.

6. Cortvrindt R, Smitz J. Early preantral mouse follicle in vitro maturation: oocyte growth, meiotic maturation and granulosa-cell proliferation. Theriogenology. 1998 Mar;49(4):845-59.

7. Cortvrindt RG, Hu Y, Liu J, Smitz JE. Timed analysis of the nuclear maturation of oocytes in early preantral mouse follicle culture supplemented with recombinant gonadotropin. Fertil Steril. 1998 Dec;70(6):1114-25.

8. Cortvrindt RG, Smitz JE. Fluorescent probes allow rapid and precise recording of follicle density and staging in human ovarian cortical biopsy samples. Fertil Steril. 2001 Mar;75(3):588-93.

9. Anckaert E, Mees M, Schiettecatte J, Smitz J. Clinical validation of a fully automated 17beta-estradiol and progesterone assay (VIDAS) for use in monitoring assisted reproduction treatment. Clin Chem Lab Med. 2002 Aug;40(8):824-31.

10. Hu Y, Cortvrindt R, Smitz J. Effects of aromatase inhibition on in vitro follicle and oocyte development analyzed by early preantral mouse follicle culture. Mol Reprod Dev. 2002 Apr;61(4):549-59.

11. Nogueira D, Albano C, Adriaenssens T, Cortvrindt R, Bourgain C, Devroey P, et al. Human oocytes reversibly arrested in prophase I by phosphodiesterase type 3 inhibitor in vitro. Biol Reprod. 2003 Sep;69(3):1042-52.

12. Nogueira D, Cortvrindt R, De Matos DG, Vanhoutte L, Smitz J. Effect of phosphodiesterase type 3 inhibitor on developmental competence of immature mouse oocytes in vitro. Biol Reprod. 2003 Dec;69(6):2045-52.

13. Adriaens I, Cortvrindt R, Smitz J. Differential FSH exposure in preantral follicle culture has marked effects on folliculogenesis and oocyte developmental competence. Hum Reprod. 2004 Feb;19(2):398-408.

14. Lenie S, Cortvrindt R, Adriaenssens T, Smitz J. A reproducible two-step culture system for isolated primary mouse ovarian follicles as single functional units. Biol Reprod. 2004 Nov;71(5):1730-8.

15. Reynaud K, Cortvrindt R, Verlinde F, De Schepper J, Bourgain C, Smitz J. Number of ovarian follicles in human fetuses with the 45,X karyotype. Fertil Steril. 2004 Apr;81(4):1112-9.

16. Smitz J. Oocyte developmental competence after heterotopic transplantation of cryopreserved ovarian tissue. Lancet. 2004 Mar 13;363(9412):832-3.

17. Vanhoutte L, Cortvrindt R, Nogueira D, Smitz J. Effects of chilling on structural aspects of early preantral mouse follicles. Biol Reprod. 2004 Apr;70(4):1041-8.

18. Nogueira D, Cortvrindt R, Everaerdt B, Smitz J. Effects of long-term in vitro exposure to phosphodiesterase type-3 inhibitors on follicle and oocyte development. Reproduction. 2005 Aug;130(2):177-86.

19. Adriaens I, Jacquet P, Cortvrindt R, Janssen K, Smitz J. Melatonin has dose-dependent effects on folliculogenesis, oocyte maturation capacity and steroidogenesis. Toxicology. 2006 Dec 7;228(2-3):333-43.

20. Nogueira D, Friedler S, Schachter M, Raziel A, Ron-El R, Smitz J. Oocyte maturity and preimplantation development in relation to follicle diameter in gonadotropin-releasing hormone agonist or antagonist treatments. Fertil Steril. 2006 Mar;85(3):578-83.

21. Nogueira D, Ron-El R, Friedler S, Schachter M, Raziel A, Cortvrindt R, et al. Meiotic arrest in vitro by phosphodiesterase 3-inhibitor enhances maturation capacity of human oocytes and allows subsequent embryonic development. Biol Reprod. 2006 Jan;74(1):177-84.

22. Sadeu JC, Cortvrindt R, Ron-El R, Kasterstein E, Smitz J. Morphological and ultrastructural evaluation of cultured frozen-thawed human fetal ovarian tissue. Fertil Steril. 2006 Apr;85 Suppl 1:1130-41.

23. Sadeu JC, Adriaens I, Cortvrindt R, Smitz J. Study of folliculogenesis in vivo in guinea pig. Theriogenology. 2007 Dec;68(9):1228-39.

24. Lenie S, Cortvrindt R, Eichenlaub-Ritter U, Smitz J. Continuous exposure to bisphenol A during in vitro follicular development induces meiotic abnormalities. Mutat Res. 2008 Mar 12;651(1-2):71-81.

25. Lenie S, Smitz J. Estrogen receptor subtypes localization shifts in cultured mouse ovarian follicles. Histochem Cell Biol. 2008 Jun;129(6):827-40.

26. Sadeu JC, Adriaenssens T, Smitz J. Expression of growth differentiation factor 9, bone morphogenetic protein 15, and anti-Mullerian hormone in cultured mouse primary follicles. Reproduction. 2008 Aug;136(2):195-203.

27. Sadeu JC, Smitz J. Growth differentiation factor-9 and anti-Mullerian hormone expression in cultured human follicles from frozen-thawed ovarian tissue. Reprod Biomed Online. 2008 Oct;17(4):537-48.

28. Segers I, Adriaenssens T, Coucke W, Cortvrindt R, Smitz J. Timing of nuclear maturation and postovulatory aging in oocytes of in vitro-grown mouse follicles with or without oil overlay. Biol Reprod. 2008 May;78(5):859-68.

29. Adriaenssens T, Mazoyer C, Segers I, Wathlet S, Smitz J. Differences in collagen expression in cumulus cells after exposure to highly purified menotropin or recombinant follicle-stimulating hormone in a mouse follicle culture model. Biol Reprod. 2009 May;80(5):1015-25.

30. Anckaert E, Adriaenssens T, Romero S, Dremier S, Smitz J. Unaltered imprinting establishment of key imprinted genes in mouse oocytes after in vitro follicle culture under variable follicle-stimulating hormone exposure. Int J Dev Biol. 2009;53(4):541-8.

31. Anckaert E, Adriaenssens T, Romero S, Smitz J. Ammonium accumulation and use of mineral oil overlay do not alter imprinting establishment at three key imprinted genes in mouse oocytes grown and matured in a long-term follicle culture. Biol Reprod. 2009 Oct;81(4):666-73.

32. Lenie S, Smitz J. Functional AR signaling is evident in an in vitro mouse follicle culture bioassay that encompasses most stages of folliculogenesis. Biol Reprod. 2009 Apr;80(4):685-95.

33. Lenie S, Smitz J. Steroidogenesis-disrupting compounds can be effectively studied for major fertility-related endpoints using in vitro cultured mouse follicles. Toxicol Lett. 2009 Mar 28;185(3):143-52.

34. Romero S, Smitz J. Epiregulin can effectively mature isolated cumulus-oocyte complexes, but fails as a substitute for the hCG/epidermal growth factor stimulus on cultured follicles. Reproduction. 2009 Jun;137(6):997-1005.

35. Sánchez F, Adriaenssens T, Romero S, Smitz J. Quantification of oocyte-specific transcripts in follicle-enclosed oocytes during antral development and maturation in vitro. Mol Hum Reprod. 2009 Sep;15(9):539-50.

36. Adriaenssens T, Wathlet S, Segers I, Verheyen G, De Vos A, Van der Elst J, et al. Cumulus cell gene expression is associated with oocyte developmental quality and influenced by patient and treatment characteristics. Hum Reprod. 2010 May;25(5):1259-70.

37. Anckaert E, Romero S, Adriaenssens T, Smitz J. Effects of low methyl donor levels in culture medium during mouse follicle culture on oocyte imprinting establishment. Biol Reprod. 2010 Sep;83(3):377-86.

38. Romero S, Smitz J. Exposing cultured mouse ovarian follicles under increased gonadotropin tonus to aromatizable androgens influences the steroid balance and reduces oocyte meiotic capacity. Endocrine. 2010 Oct;38(2):243-53.

39. Romero S, Smitz J. Improvement of in vitro culture of mouse cumulus-oocyte complexes using PDE3-inhibitor followed by meiosis induction with epiregulin. Fertil Steril. 2010 Feb;93(3):936-44.

40. Sánchez F, Adriaenssens T, Romero S, Smitz J. Different follicle-stimulating hormone exposure regimens during antral follicle growth alter gene expression in the cumulus-oocyte complex in mice. Biol Reprod. 2010 Oct;83(4):514-24.

41. Segers I, Adriaenssens T, Ozturk E, Smitz J. Acquisition and loss of oocyte meiotic and developmental competence during in vitro antral follicle growth in mouse. Fertil Steril. 2010 May 15;93(8):2695-700.

42. Adriaenssens T, Segers I, Wathlet S, Smitz J. The cumulus cell gene expression profile of oocytes with different nuclear maturity and potential for blastocyst formation. J Assist Reprod Genet. 2011 Jan;28(1):31-40.

43. De Vos M, Ortega-Hrepich C, Albuz FK, Guzmán L, Polyzos NP, Smitz J, et al. Clinical outcome of non-hCG-primed oocyte in vitro maturation treatment in patients with polycystic ovaries and polycystic ovary syndrome. Fertil Steril. 2011 Oct;96(4):860-4.

44. Romero S, Sánchez F, Adriaenssens T, Smitz J. Mouse cumulus-oocyte complexes from in vitro-cultured preantral follicles suggest an anti-luteinizing role for the EGF cascade in the cumulus cells. Biol Reprod. 2011 Jun;84(6):1164-70.

45. Sánchez F, Romero S, Smitz J. Oocyte and cumulus cell transcripts from cultured mouse follicles are induced to deviate from normal in vivo conditions by combinations of insulin, follicle-stimulating hormone, and human chorionic gonadotropin. Biol Reprod. 2011 Sep;85(3):565-74.

46. Wathlet S, Adriaenssens T, Segers I, Verheyen G, Van de Velde H, Coucke W, et al. Cumulus cell gene expression predicts better cleavage-stage embryo or blastocyst development and pregnancy for ICSI patients. Hum Reprod. 2011 May;26(5):1035-51.

47. Sánchez F, Romero S, Albuz FK, Smitz J. In vitro follicle growth under non-attachment conditions and decreased FSH levels reduces Lhcgr expression in cumulus cells and promotes oocyte developmental competence. J Assist Reprod Genet 29, 141-152, 2012 (IF: 1.823)

48. Sánchez F, Smitz J. Molecular control of oogenesis. Biochim Biophys Acta 1822, 1896-1912, 2012

49. Polyzos NP, Stoop D, Blockeel C, Adriaensen P, Platteau P, Anckaert E, Smitz J, Devroey P. Anti-Müllerian hormone for the assessment of ovarian response in GnRH-antagonist-treated oocyte donors. Reprod Biomed Online 24, 532-539, 2012 (IF: 2.675)

50. Segers I, Adriaenssens T, Smitz J. Expression patterns of poliovirus receptor, erythrocyte protein band 4.1 like 3, regulator of G-protein signaling 11 and oxytocin receptor in mouse ovarian cells during follicle growth and early luteinisation in vitro and in vivo. Biol Reprod 86, 1-11, 2012 (IF: 4.027)

51. Anckaert E, Smitz J, Schiettecatte J, Klein BM, Arce JC. The value of anti-Müllerian hormone measurement in the long GnRH agonist protocol: assocation with ovarian response, embryo quality, pregnancy and dose adjustments. Hum Reprod 27, 1829-1839, 2012 (IF: 4.670)

52. Guzmán L, Ortega-Hrepich C, Albuz FK, Verheyen G, Devroey P, Smitz J, De Vos M. Developmental capacity of in vitro matured human oocytes retrieved from polycystic ovary syndrome ovaries containing no follicles larger than 6 mm. Fertil Steril 98, 503-507, 2012 (IF: 4.174)

53. Donnez J, Jadoul P, Pirard C, Hutchings G, Demylle D, Squifflet J, Smitz J, Dolmans MM. Live birth after transplantation of frozen-thawed ovarian tissue after bilateral oophorectomy for benign disease. Fertil Steril 98, 720-725, 2012 (IF: 4.174)

54. Wathlet S, Adriaenssens T, Segers I, Verheyen G, Janssens R, Coucke W, Devroey P, Smitz J. New candidate genes to predict pregnancy outcome in single embryo transfer cycles when using cumulus cell gene expression. Fertil Steril 98, 432-439, 2012 (IF: 4.174)

55. Thuesen LL, Loft A, Egeberg AN, Smitz J, Petersen JH, Nyboe Andersen A. A randomized controlled dose-response pilot study of adding hCG to recombinant FSH during controlled ovarian stimulation for in vitro fertilization. Hum Reprod 27, 3074-3084, 2012 (IF: 4.670)

56. Segers I, Adriaenssens T, Wathlet S, Smitz J. Gene expression differences induced by equimolar low doses of LH or hCG in combination with FSH in cultured mouse antral follicles. J Endocrinol 215, 269-280, 2012 (IF: 4.058)

57. Sanfilippo S, Canis M, Romero S, Sion B, Déchelotte P, Pouly JL, Janny L, Smitz J, Brugnon F. Quality and functionality of human ovarian tissue after cryopreservation using an original slow freezing procedure. J Assist Reprod Genet 30, 25-34, 2013 (IF: 1.823)

58. Arce JC and Smitz J. Live birth rates after HP-hMG stimulation in the long GnRH agonist protocol: association with mid-follicular hCG and progesterone concentrations, but not with LH concentrations. Gynecol Endocrinol 29, 46-50, 2013 (IF: 1.303)

59. Guzmán L, Adriaenssens T, Ortega-Hrepich C, Albuz FK, Mateizel I, Devroey P, De Vos M, Smitz J. Human antral follicles < 6 mm: a comparison between in vivo maturation and in vitro maturation in non-hCG primed cycles using cumulus cell gene expression. Mol Hum Reprod 19, 7-16, 2013 (IF: 4.542)

60. Anckaert E, De Rycke M, Smitz J. Culture of oocytes and risk of imprinting defects. Hum Reprod Update 19, 52-66, 2013 (IF: 8.847)

61. Thuesen LL, Loft A, Smitz J, Nyboe Andersen A. Reply: HCG supplementation of controlled ovarian stimulation cycles. Hum Reprod 28, 284, 2013 (IF: 4.670)

62. Alves AM, Chaves R, Rocha R, Ferreira de Lima L, Andrade PM, Lopes C, Souza C, Moura A, Campello C, Bao S, Smitz J, de Figueiredo JR. Dynamic medium containing Growth Differentiation Factor 9 (GDF-9) and follicle-stimulating hormone (FSH) maintains survival and promotes in vitro growth of caprine preantral follicles after long-term in vitro culture. Reprod Fertil Dev 25:955-965, 2013 (IF: 2.583)

63. Guzmán L, Ortega-Hrepich C, Polyzos NP, Anckaert E, Verheyen G, Coucke W, Devroey P, Tournaye H, Smitz J, De Vos M. A prediction model to select PCOS patients suitable for IVM treatment based on anti-Müllerian hormone and antral follicle count. Hum Reprod 28, 1261-1266, 2013 (IF: 4.670)

64. Thuesen LL, Smitz J, Loft A, Nyboe Andersen A. Endocrine effects of hCG supplementation to recombinant FSH throughout controlled ovarian stimulation for IVF: a dose response study. Clin Endocrinol 79, 708-715, 2013 (IF: 3.396)

65. Zeng H, Ren Z, Guzman L, Wang X, Sutton-McDowall ML, Ritter LJ, De Vos M, Smitz J, Thompson JG, Gilchrist RB. Heparin and cAMP modulators interact during pre-in vitro maturation to affect mouse and human oocyte meiosis and developmental competence. Hum Reprod 28, 1536-1545, 2013 (IF: 4.670)

66. Fleming R, Broekmans F, Calhaz-Jorge C, Dracea L, Alexander H, Nyboe Andersen A, Blockeel C, Jenkins J, Lunenfeld B, Platteau P, Smitz J, de Ziegler D. Can anti-Müllerian hormone concentrations be used to determine gonadotrophin dose and treatment protocol for ovarian stimulation? Reprod Biomed Online 26, 431-439, 2013 (IF: 2.675)

67. Wathlet S, Adriaenssens T, Segers I, Verheyen G, Van Landuyt L, Coucke W, Devroey P, Smitz J. Pregnancy prediction in single embryo transfer cycles after ICSI using QPCR: validation in oocytes from the same cohort. PLoS One 8, e54226, 2013 (IF: 3.730)

68. Stoop D, Anckaert E, Smitz J. Safety of ovarian stimulation. In: Trounson A, Gosden R, Eichenlaub-Ritter U (eds). Biology and Pathology of the Oocyte: Role in Fertility, Medicine and Nuclear Reprogramming, 2nd Edition. Cambridge University Press, 2013

69. Ortega-Hrepich C, Stoop D, Guzman L, Van Landuyt L, Tournaye H, Smitz J, De Vos M. A "freeze-all" embryo strategy after in vitro maturation: a novel approach in women with polycystic ovary syndrome? Fertil Steril 100, 1002-1007, 2013 (IF: 4.174)

70. Anckaert E, Sanchez F, Billooye K, Smitz J. Dynamics of imprinted DNA methylation and gene transcription for imprinting establishment in mouse oocytes in relation to culture duration variability. Biol Reprod 89, 130:1-8, 2013 (IF: 4.027)

71. Nguyen DN, Huyghens L, Wellens F, Schiettecatte J, Smitz J, Vincent JL. Serum S100B protein could help to detect cerebral complications associated with extracorporeal membrane oxygenation (ECMO). Neurocrit Care 20, 367-374, 2014 (IF: 3.038)

72. Choi J and Smitz J. Luteinizing hormone and human chorionic gonadotropin: origins of difference. Mol Cell Endocrinol 383, 203-213, 2014 (IF: 4.039)

73. Choi J and Smitz J. Luteinizing hormone and human chorionic gonadotropin: distinguishing unique physiologic roles. Gynecol Endocrinol 30, 174-181, 2014 (IF: 1.303)

74. Thuesen LL, Andersen AN, Loft A, Smitz J. Intrafollicular milieu after addition of hCG to recombinant FSH during controlled ovarian stimulation for in vitro fertilization. J Clin Endocrinol Metab 99, 517-526, 2014 (IF: 6.430)

75. Ortega-Hrepich C, Polyzos NP, Anckaert E, Guzman L, Tournaye H, Smitz J, De Vos M. The effect of ovarian puncture on the endocrine profile of PCOS patients who undergo IVM. Reprod Biol Endocr 12, 18, 2014 (IF: 2.144)

76. Sanfilippo S, Canis M, Smitz J, Sion B, Darcha C, Janny L, Brugnon F. Comparison of vitrification versus slow freezing techniques on human ovarian tissue quality. Eur J Obstet Gynecol, submitted 2013 (IF: 1.843)

77. Nguyen DN, Huyghens L, Zhang H, Schiettecatte J, Smitz JEJ, Vincent JL. Cortisol is an associated-risk factor of brain dysfunction in patients with severe sepsis and septic shock. BioMed Res Int in press 2014

78. Richani D, Wang X, Zeng H, Smitz JEJ, Thompson JG, Gilchrist RB. Pre-maturation with cAMP modulators in conjunction with EGF-like peptides during in vitro maturation enhances mouse oocyte developmental competence. Mol Reprod Dev 81, 422-435, 2014 (IF: 2.812)

79. Kuhtz J, Romero S, De Vos M, Smitz J, Haaf T, Anckaert E. Human in vitro oocyte maturation is not associated with increased imprinting error rates at LIT1, SNRPN, PEG3 and GTL2. Hum Reprod 29, 1995-2005, 2014 (IF: 4.585)

80. Polyzos NP, Anckaert E, Guzman L, Schiettecatte J, Van Landuyt L, Camus M, Smitz J, Tournaye H. Vitamin D deficiency and pregnancy rates in women undergoing single embryo, blastocyst stage, transfer (SET) for IVF/ICSI. Hum Reprod 29, 2032-2040, 2014 (IF: 4.585)

81. Santos-Ribeiro S, Polyzos N, Haentjens P, Smitz J, Camus M, Tournaye H, Blockeel C. Live-birth rates after IVF are reduced by both low and high progesterone levels on the day of human chorionic gonadotrophin administration. Hum Reprod 29, 1698-1705, 2014 (IF: 4.585)

82. Zeng H, Richani D, Sutton-McDowall ML, Ren Z, Smitz JEJ, Stokes Y, Gilchrist RB, Thompson JG. Pre-maturation with cyclic adenosine monophosphate modulators alters cumulus cell and oocyte metabolism and enhances developmental competence of in vitro matured mouse oocytes. Biol Reprod 91, 47, 2014 (IF: 4.027)

83. De Vos M, Smitz J, Woodruff TK. Fertility preservation in female cancer patients. Lancet 384, 1302-1310, 2014 (IF: 39.207)

84. Ortega-Hrepich C, Schwarze J-E, Guzman L, Tournaye H, Smitz J, De Vos M. Identification of parameters predicting pain scores in patients undergoing oocyte retrieval for IVM. Fertil Steril, in press 2014

85. Spits C, Guzman L, Mertzanidou A, Jacobs K, Ortega-Hrepich C, Gilchrist RB, Thompson JG, De Vos M, Smitz J, Sermon K. Chromosome constitution of human embryos generated after in vitro maturation (IVM) including 3-isobutyl-1-methylxanthine (IBMX) in oocyte collection medium. Hum Reprod 30, 653-663, 2015

86. Anderson RA, Anckaert E, Bosch E, Dewailly D, Dunlop CE, Fehr D, Nardo L, Smitz J, Tremellen K, Denk B, Geistanger A, Hund M. A prospective study into the value of the Elecsys AMH assay for the assessment of the ovarian growing follicle pool. Fertil Steril 103, 1074-1080, 2015

87. Sanfilippo S, Canis M, Smitz J, Sion B, Darcha C, Janny L, Brugnon F. Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing? Reprod Biol Endocrinol 13, 67, 2015

88. Gilchrist RB, Zeng HT, Wang X, Richani D, Smitz J, Thompson JG. Re-evaluation and evolution of the simulated physiological oocyte maturation (SPOM) system. Theriogenology, pii: S0093-691X(15)00161-2, 2015

89. Segers I, Mateizel I, Van Moer E, Smitz J, Tournaye H, Verheyen G, De Vos M. In vitro maturation (IVM) of oocytes recovered from ovariectomy specimens in the laboratory: a promising "ex vivo" method of oocyte cryopreservation resulting in the first report of an ongoing pregnancy in Europe. J Assist Reprod Genet 2015, submitted

90. Sugimura S, Ritter LJ, Rose RD, Thompson JG, Smitz J, Mottershead DG, Gilchrist RB. Promotion of EGF receptor signaling improves the quality of low developmental competence oocytes. Dev Biol 403, 139-149, 2015

91. Sánchez F, Romero S, De Vos M, Verheyen G, Smitz J. Human cumulus-enclosed germinal vesicle oocytes from early antral follicles reveal heterogeneous cellular and molecular features associated with in vitro maturation capacity. Hum Reprod 30, 1396-1409, 2015



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