Vrije Universiteit Brussel






Mouse Follicle Culture

In vitro models for culturing ovarian follicles allow for the study of factors and environmental conditions that influence the development and differentiation of the ovarian follicle and determine oocyte and embryo quality. Due to the accessibility to the different growth stages under in vitro situations, it also provides a suitable model for the study of basic aspects of oocyte and follicle biology at the cellular and molecular levels.

The advantage of the rodent model is that the follicular cycle is relatively short, which makes it feasible to be entirely reproduced in vitro.

FOBI has developed a mouse follicle culture system that allows to grow follicles from the secondary stage to the fully antral stage, where the oocyte reaches maturity and can be fertilized.

Because the knowledge on ovarian physiology is continuously growing, researchers at FOBI translate this knowledge into improvements of the culture system, and therefore it is constantly evolving.


Pictures of mouse follicles in culture in an attaching permissive system.


Picture Galery

Viable primordial follicles (green) within frozen-thawed human ovarian tissue.



Mouse ovarian follicle after 8 days of culture, stained with Texas red phalloidin (red) and sybr green (green).


 

Follicle Biology Unit • ©2012 • http://fobi.vub.ac.be
VUB • Faculty of Medicine & Pharmacy • Laarbeeklaan 103 • B-1090 Brussel, Belgium
Tel: +32 (0)2 477 50 50 • Fax: +32 (0)2 477 50 60 • Sandra.DeSchaepdryver@uzbrussel.be
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